How much do you know about traditional cell culture technology?

  • Cell culture

    Cell culture is also called cell cloning technology, also known as cell culture technology. Cell culture is an indispensable process for whole bioengineering technology and bioclone technology. Cell culture itself is a large-scale cloning of cells. Cells can be cultured from one cell to a simple single cell or a very small number of differentiated cells, which is an essential part of cloning technology, and cell culture itself is a clone of cells. A large number of cells or their metabolites are obtained by cell culture. Because biological products are derived from cells, it can be said that cell culture technology is the core and most basic technology in biotechnology.

     

     

    Different cell culture conditions:

    Folding animal cell culture: In all cell cultures in vitro, the most difficult is animal cell culture. (1) Serum: Serum is often required for in vitro culture of animal cells. The most commonly used is calf serum. Serum provides essential growth factors such as hormones, trace elements, minerals and fats. Serum is equal to the natural nutrient solution cultured in vitro by animal cells. (2) Support: Most animal cells have the habit of adherent growth. In vitro culture commonly used glass, plastic, etc. as a support. (3) Gas exchange: The ratio of carbon dioxide and oxygen should be continuously adjusted during the cell culture process to continuously maintain the required gas conditions; thus, it is determined that the animal cell in vitro culture equipment requires high investment and large investment.

     

    Folding plant cell culture: (1) Illumination: Plant cells cultured in vitro are not very strict with light conditions, because the substances required for cell growth are mainly supplied by the medium. However, light is not only related to photosynthesis, but also related to cell differentiation; in the process of obtaining important substances such as drugs by plant cells in vitro, plant cells are mostly cultured in suspension in a reactor. (2) Hormones: The division and growth of plant cells require the regulation of plant hormones. The auxin that promotes growth and the mitogen that promotes cell division are the most basic hormones. Plant cell division, growth, differentiation and individual growth cycle have corresponding hormones involved in regulation. Compared with animal cells, the principle of hormone requirements for plant cell culture in vitro has been well understood, and its application technology is quite mature. There is already a set of culture fluids widely used as commodities. At the same time, the plant cells are required for water, nutrients, hormones, osmotic pressure, pH, and trace elements.

     

    Microbial cell culture: microorganisms are mostly single-celled organisms, and the wild living conditions are relatively simple. Therefore, the conditions for artificial culture of microorganisms are much simpler than those of animal and plant cells. Among them, anaerobic microbial culture is more complicated than aerobic microorganisms, because strict anaerobic needs to maintain a non-oxygen inert gas concentration such as carbon dioxide, while aerobic microorganisms only need to provide sterile oxygen through continuous stirring. Microorganisms are less demanding on culture conditions than animal and plant cells, and corn syrup, peptone, wort, yeast extract, etc. become good microbial natural media. For the nutritional requirements of some special microorganisms, additional additions can be made on the basis of these natural media.

     

    So, what are the advantages and disadvantages of cell culture? Advantages: 1. It can be passaged for a long time, maintains activity, and is convenient for monitoring and detecting structural functions and life activities. 2, culture conditions can be artificially controlled to study the effects of cellular metabolism, physical, chemical, biological factors 3, can be used for a variety of observations and detection methods to study changes in living cells (variation, differentiation). The expression of subcellular structures and metabolic molecules can be carried out at the cellular level. 4. The research scope and source of cells are wide and the selectivity is wide. 5. Different generations of cells can be preserved for a long time. It is possible to carry out research on different conditions and methods of the same generation, and to observe the dynamic changes of different generations.6, low cost, economy, low cost, can be cultivated in large quantities, which is conducive to the production of biological products. Disadvantages: Cells or tissues grow in an artificial environment, and there are differences in the environment of the body, and the morphology or function of cells or tissues may change to varying degrees.

    Research and application of cell culture technology

    1. Directly observe the morphological structure and life activities of living cells. Used in cytology, genetics, immunology, experimental medicine and oncology.
    2. Direct observation of changes in cells can facilitate photography.
    3. Study cell types such as low to high, human, embryo to adult, normal tissue to tumor.
    4. Easy to use a variety of techniques: phase difference, fluorescence, electron microscopy, histochemistry, isotope labeling and other methods to observe and study cell conditions.
    5. It is the research object of molecular biology and genetic engineering, and it is also its main component.
    6. Easy to apply experimental studies of physical and chemical organisms.
    7. It is easy to provide a large number of experimental subjects with similar biological traits, and it is less expensive and economical.
    8. Become a source of materials for bioproduct monoclonal antibody production and genetic engineering.

    However, the shortcoming is that tissues and cells survive independently in an artificial culture environment, although the internal environment is still very different. Therefore, when using cultured cells for experiments, it should not be considered that the cells in the body are identical, and the results of the experiments are presumed to be in vivo, and it is easy to make conclusions equivalent to the body.

     

    What is the difference between traditional cell culture techniques and 3D cell culture?

    3D cell culture is a culture technique that provides cells with a microenvironment closer to the living conditions in vivo during cell culture. Traditional cell culture techniques have not been sufficient to mimic the living environment of cells. The invention of 3D cell culture technology is to provide cells with a microenvironment closer to the living conditions in vivo during cell culture.

    The principle is to use magnetic microsphere carrier and magnetic suspension technology to suspend the cell-attached microsphere carrier in the culture solution, ensuring high-quality, high-density cell reproduction, breaking through the traditional covered culture dish, culture flask or microplate cell. The cultivation is time-consuming and cumbersome, and the cell yield is small and other limitations.